Centre Matias Moroni will miss the cut after sidelined with a knee ligament injury allowing a recall for Racing 92 bound Juan Imhoff, who returns for his first game since 2015 World Cup.
Earlier, last month a number of positive cases were reported in Argentina camp including Ledesma but now they will be looking to overcome the setback as they are set to play their first international game of the year.
Argentina players currently are in Uruguay for a training camp and are expected to leave to Australia on Sunday with the 13 Europe bound players in the squad expected to join them on 19th October. All the players and staff will have to undergo a mandatory 14-day quarantine period in Sydney.
Argentina will kick-start their Rugby Championship tournament with a clash against South Africa on 7th November at Lang Park in Brisbane.
Argentina 45-man squad:
Tomas Albornoz, Matías Alemanno, Gonzalo Bertranou, Emiliano Boffelli, Facundo Bosch, Rodrigo Bruni, Ignacio Calas, Ignacio Calles, Sebastian Cancelliere, Santiago Carreras, Santiago Chocobares, Lucio Cinti, Santiago Cordero, Tomas Cubelli, Jeronimo De la Fuente, Bautista Delguy, Joaquín Díaz Bonilla, Felipe Ezcurra, Rodrigo Fernández Criado, José Luis Gonzalez, Juan Martin Gonzalez, Francisco Gorrissen, Santiago Grondona, Juan Imhoff, Facundo Isa, Marcos Kremer, Tomas Lezana, Juan Cruz Mallia, Pablo Matera, Santiago Medrano, Domingo Miotti, Julian Montoya, Ramiro Moyano, Matias Orlando, Joaquin Oviedo, Lucas Paulos, Guido Petti, Enrique Pieretto, Nicolas Sanchez, Santiago Socino, Lucio Sordoni, Nahuel Tetaz Chaparro, Mayco Vivas, Federico Wegrzyn, Juan Pablo Zeiss.