England and Leicester Tigers fly-half George Ford has pulled out of the Quilter Cup clash against Barbarians following Achilles tendon injury.
Bristol Bears trio Ben Earl, Max Malins and Kyle Sinckler have been added to the squad following their recent triumph in the European Challenge Cup.
There are 11 uncapped players in squad with five including Ali Crossdale, Fraser Dingwall, Alex Mitchell, Ollie Lawrence and Gloucester wing Ollie Thorley in the backs and Alex Dombrandt, Tom Dunn, Alex Moon, David Ribbans, and Joe Heyes in the forwards.
Players from Exeter Chiefs and Wasps, who will be part of the Gallagher Premiership final at Twickenham Stadium on Saturday have been omitted from the squad.
Following the game against Barbarians, England will face Italy in the final round of the Six Nations tournament on 31st October before taking part in the Autumn Nations Cup.
Backs: Ali Crossdale (Saracens), Fraser Dingwall (Northampton Saints), Owen Farrell (Saracens), Piers Francis (Northampton Saints), George Furbank (Northampton Saints), Willi Heinz (Gloucester), Jonathan Joseph (Bath), Ollie Lawrence (Worcester Warriors), Max Malins (Bristol Bears), Joe Marchant (Harlequins), Jonny May (Gloucester), Alex Mitchell (Northampton Saints), Ollie Thorley (Gloucester), Anthony Watson (Bath), Ben Youngs (Leicester Tigers)
Forwards: Tom Curry (Sale Sharks), Alex Dombrandt (Harlequins), Tom Dunn (Bath), Ben Earl (Bristol Bears), Charlie Ewels (Bath), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Joe Heyes (Leicester Tigers). Ted Hill (Worcester Warriors), Maro Itoje (Saracens), Alex Moon (Northampton Saints), Beno Obano (Bath), David Ribbans (Northampton Saints), Kyle Sinckler (Bristol Bears), Will Stuart (Bath), Sam Underhill (Bath), Billy Vunipola (Saracens), Mako Vunipola (Saracens), Mark Wilson (Newcastle Falcons)