Amy Cokayne to miss England's Six Nations game against Wales

England will miss the services of Amy Cokayne for the third round of the Women's Six Nations against Wales to be played on Saturday.

Cokayne will be representing the Royal Air Force for a clash against the British Army at Kingsholm with her place taken by Lark Davies.

"The RAF ask very little of us and we get an awful lot from them. It is a really good relationship, there is a massive amount of respect between both parties," said Middleton.

"Amy gets afforded to train with us, play with us, pretty much unlimited and they have a fixture or a couple of fixtures each year that we release Amy for. Amy is really passionate about playing for England but equally as passionate about being in the RAF."

Emma Sing will make her first international start as she is slotted at fullback with Abby Dow switching to left wing at the expense of Claudia McDonald.

The final change sees Alex Matthews making her first appearance of the tournament at number eight with Zoe Aldcroft shifting to second row joining Cath O'Donnell.

May Campbell could make her England debut when he she comes off the bench that also has Hannah Botterman, Maud Muir and Ellie Kildunne returning.

"Emma Sing makes her first start in an England shirt. She's performed extremely well for Gloucester-Hartpury this season and has impressed us in camps and her international appearances to date," he added.

"It is a great opportunity to give Emma a start in a 15 shirt and let her do her thing. I don't think we lose much with Abby [Dow] going on the wing."

England: 15 Emma Sing, 14 Jess Breach, 13 Lagi Tuima, 12 Tatyana Heard, 11 Abby Dow, 10 Holly Aitchison, 9 Lucy Packer, 1 Mackenzie Carson, 2 Lark Davies, 3 Sarah Bern, 4 Zoe Aldcroft (vice-captain), 5 Cath O'Donnell, 6 Sadia Kabeya, 7 Marlie Packer (captain), 8 Alex Matthews

Replacements: 16 May Campbell, 17 Hannah Botterman, 18 Maud Muir, 19 Delaney Burns, 20 Sarah Beckett, 21 Ella Wyrwas, 22 Sarah McKenna, 23 Ellie Kildunne